Stem cells and biomaterials

Stem cell research is one of the most rapidly developing areas of biomedicine. Recent stem cell technologies have opened up several novel avenues for biomedical research, such as developing disease models, drug development, tissue regeneration and development of functional organoids.

In this BF network efforts are directed to obtain knowledge and protocols to generate stem cells from different sources. The network aims also at development of adult stem cell-based tissue engineered biomaterial implants and organoids.

A special emphasis is put to develop techniques to generate and use the so called induced pluripotent cells (iPS) from committed permanently differentiated cells. The discovery that somatic cells can be reprogrammed into pluripotency via only three developmental control genes has opened new horizons for stem cells in e.g. derivation of patient specific cellular models for basic and applied research. Propagation of pluripotent cells from patients permit for the first time detailed studies on the molecular biology of human disease mechanisms and the use of such cells for development of novel therapeutics. In the long term for the iPS cells should provide a unique way to develop technologies for obtaining immunologically tolerated cells for cell and tissue transplantation.

The main challenges of the network are: 1) how to channel and validate stem cells to specific cell lineages and functional cell types, 2) how to use these in tissue engineering and regeneration, and 3) how to use these as models for drug screening and organoid development.

Contact persons & Feedback

Technology platform services

Provision of human pluripotent cells

BF network offers a large range of human embryonic (hESCs) and induced pluripotent stem cell (iPS cells) lines with a complete service package including protocols for the stem cell maintenance and expansion, characterization data documentation and highly qualified technical support. BF network can assist with the selection of the appropriate stem cell line for your specific research needs.

Service providers

Helsinki
Biomedicum Stem Cell Center

Tampere
BioMediTech Facility

Kuopio
Stem Cell Center

Generation of iPS cells from fibroblast cell line provided by client

BF network offers custom iPS cell line production service. Currently the methods for iPS cell generation are based on retro-or lentiviral transduction, but non-viral methods are under development. Genetically modified iPS cell lines can be developed according to customer's needs.

Service providers

Helsinki
Biomedicum Stem Cell Center

Tampere:
BioMediTech Facility

Derivation of stem cell lines in GMP conditions

BF network can provide the establishment of high-quality GMP-compatible hESC and iPS cell lines. These cell lines will be produced by trained staff in clean room facility using high-quality materials and reagents.

Service providers

Tampere:
 • BioMediTech Facility

Validation of pluripotent stem cells

BF network provides extensive range of characterization options for stem cells including: phenotype, genotype, growth and differentiation characteristics.

Service providers

Helsinki:
Biomedicum Stem Cell Center

Tampere:
BioMediTech Facility

Protocol development and biomaterials based screening towards differentiation of stem cells/iPS cells into different lineages

BF network provides protocols and protocol development for differentiation of stem cells/iPS cells into different lineages. Supply of other differentiated cell types can be negotiated.

- Hepatic, pancreatic, neural. Contact: Biomedicum Stem Cell Center, Helsinki
- Cardiomyocytes, retinal, neural. Contact: BioMediTech Facility, Tampere
- Mesodermal, neural. Contact: Biocenter Kuopio, Stem Cell Center

Biomaterials, nanoparticles and matrix components

BF network offers biomaterials, nanoparticles and matrix components. End-points and differentiation assays are developed with customer.
Contact: Marjo Yliperttula, Univ. Helsinki, Faculty of Pharmacy.

Plasmids in HT system

BF network offers plasmids in HT system for optimal gene transfection or silencing (miRNA, siRNA) expertise and facility. Contact: Marjo Yliperttula, Univ. Helsinki, Faculty of Pharmacy

Functional characterization

BF network offers a range of functional characterization assays.

- Cellular electrophysiology: membrane ion channels, membrane functions, single cell responses. Contact: BioMediTech Facility, Tampere; Biocenter Kuopio  Stem Cell Center
- Cell culture arrays and multi-well plates with biomaterials. Plates and spotted arrays with sets of biomaterials and bioactive compounds for stem cell differentiation experiments. Contact: Marjo Yliperttula, Univ. Helsinki, Faculty of Pharmacy
- Chondrogenic and osteogenic differentiation: qRT-PCR, ECM analysis, immunocytochemistry. Contact: Biocenter Kuopio Stem Cell Center

Training in human pluripotent stem cells technologies

BF network offers hands on laboratory-based training in fundamental aspects of pluripotent stem cell culture and analysis.

Service providers

- Training packages tailored for the client's needs in stem cell culture and iPS cell derivation. Contact: Biomedicum Stem Cell Center, Helsinki
- Custom made programs on GMP/GLP cell production. Contact: BioMediTech Facility, Tampere
- Training on biomaterial cell culture arrays and non-viral transfection with biomaterials. Contact: Marjo Yliperttula, Univ. Helsinki, Faculty of Pharmacy

Contact persons & Feedback

Stem cells and biomaterials technology platform user survey

Stem cells and biomaterials technology platform user survey

Contact persons

Coordinator of the BF Stem cells and biomaterials network
Timo Otonkoski / timo.otonkoski (at) helsinki.fi / tel: +358 2941 25692
University of Helsinki, Biocentrum Helsinki
Biomedicum Stem Cell Center

BF Stem cells and biomaterials technology platform partners:
Timo Otonkoski, BCH, Biomedicum Stem Cell Center (BSCC)(chair of the consortium); Katriina Aalto-Setälä, BMT; Marjo Yliperttula, Faculty of Pharmacy, University of Helsinki; Jari Koistinaho, BCK, Stem Cell Center