New instrument available for purification of high quality samples for structural and functional studies
Biocomplex (Structural Biology platform) offers a new instrument, a new generation asymmetrical flow field-flow fractionation (AF4) instrument NEON Eclipse (Wyatt), that is coupled to five online detectors, for use in your research projects on large macromolecular complexes.
Asymmetrical flow field-flow fractionation (AF4) is a versatile and gentle size-based separation method that is well suited for biological macromolecules and their complexes, membrane vesicles, and nanoparticles (viruses, virus-like particles etc.). It separates sample components according to size in an open channel. This leads to large separation range, from 1 to 1000 nm in radius, and gentle separation.
With the support of the Academy of Finland and the Faculty of Biological and Environmental Sciences, Biocomplex unit has acquired a new generation asymmetrical flow field-flow fractionation (AF4) instrument NEON Eclipse (Wyatt) that is coupled to five online detectors: DAWN multi-angle light scattering detector (MALS) with 18 angles; QELS dynamic light scattering (DLS) detector; fluorescence detector; UV and Optilab® differential refractive index (dRI) detectors.
The setup can be used in analytical and semi-preparative purification mode. The accompanied software (ASTRA) allows characterization of various biophysical properties of sample components: size (radius of gyration/root mean square radius and hydrodynamic radius), shape, molar mass, particle concentration, composition of conjugates, aggregation, molecular interactions. Thus, valuable information can be collected for the study of biomolecules. Fraction collection enables off-line analysis.
See also information on our other separation technologies: preparative ultracentrifugation and monolithic chromatography for large biocomplexes (Biocomplex websites)
You can apply for fully funded access to all Biocomplex services through Instruct-ERIC (Proposal guidelines).
Biocomplex is part of the Faculty of Biological and Environmental Sciences and HiLIFE at the University of Helsinki. Biocomplex is also part of a national research infrastructure network FINStruct that is the Structural Biology Platform of Biocenter Finland, and Instruct-ERIC that is a pan-European distributed research infrastructure in structural biology.
Contact person: Katri Eskelin
For more Structural Biology services, see https://www.biocenter.fi/index.php/technology-platform-services/structural-biology